SCI 252 - MEDICAL MICROBIOLOGY
Examination #1

Pts    Question

5         1. Define or provide the formula for the following items:
                a. glycine
                b. pilus
                c. peritrichous
                d. anaerobic
                e. plasmid

5          2. Provide a schematic representation of a Gram-negative bacterial cell wall.

10        3. Design an anabolic or catabolic operon.

10        4. Using transduction OR transformation OR conjugation describe how
                Staphylococcus aureus is able to acquire a resistance to an antibiotic.

10        5. Choose and diagram ONE of the following:
                    a. DNA synthesis,        OR
                    b. Protein synthesis,    OR
                    c. Glycolysis.

20        6. Choose and answer TWO of the following:
                    a. Discuss or describe the differences among sanitation, disinfection, and
                            sterilization,            OR
                    b. Describe the significance of plasmids in bacteria,    OR
 
                    c. How do bacteria prevent destruction of their own DNA?,    OR

                    d. List the processes necessary for cells to function,    OR

                    e. Describe how genetic information is stored in prokaryotic cells.

40        7. Complete/answer each of the 20 statements/questions below using these test
                        sheets.
 
                    Organisms that absorb crystal violet and are not easily decolorized by alcohol are referred
                        to as:
                                a. Gram negative
                                b. acid fast
                                c. Gram positive
                                d. pathogenic

                    Typical prokaryotic cells have their genetic material in the form of:
                                a. a single DNA strand
                                b. pairs (23) of chromosomes
                                c. several DNA fragments called the genome
                                d. a single circular double-stranded molecule of DNA

                    Which of the following environmental factors will not stimulate endospore formation?
                                a. mesophilic temperatures
                                b. freezing
                                c. aridity
                                d. boiling

                    In a rapidly multiplying bacterial population, cell numbers increase:
                                a. indirectly
                                b. arithmetically
                                c. logarithmically
                                d. longitudinally

                    All coenzymes are derivatives of:
                                a. minerals
                                b. hormones
                                c. vitamins
                                d. metabolites

                    The compound from which hydrogen atoms are removed becomes __________
                        and the NAD becomes ___________ when it accepts hydrogen atoms.
                                a. reduced, oxidized
                                b. converted, reconverted
                                c. utilized, oxidized
                                d. oxidized, reduced

                    Which of the following processes generates the greatest amount of energy?
                                a. fermentation
                                b. glycolysis
                                c. aerobic respiration
                                d. anaerobic respiration

                    In the TCA cycle, an important reaction is one in which the cell incorporates ammonia
                        into an organic molecule. One such example happens when a-ketoglutarate reacts
                        with NH4 to form:
                                a. protein
                                b. valine
                                c. glutamic acid
                                d. glucose

                    The synthesis of new strands of DNA begins at a specific site referred to as the:
                                a. origin of replication
                                b. replication fork
                                c. active site
                                d. central dogma

                    There are several mRNA sequences that do not code for any of the twenty amino acids.
                        These are called:
                                a. anti-codons
                                b. non-template codons
                                c. nonsense codons
                                d. introns

                    The specific sequence of nucleotides in the DNA to which RNA polymerase attaches
                        is termed the:
                                a. promoter region
                                b. regulatory region
                                c. sigma region
                                d. core region

                    The placement of the amino acid transferred from tRNA to mRNA is determined by the:
                                a. complementarity of nucleotides on the two molecules
                                b. need of the protein in the organism
                                c. DNA which transcribes both molecules
                                d. sequence of nucleotides on the rRNA

                    E. coli breaks down glucose with enzymes that are not subject to regulation by induction
                        or repression. Such enzymes are termed:
                                a. constitutive
                                b. template
                                c. sigma
                                d. holoenzymes

                    Segments of DNA, found in virtually all species of bacteria, that can move from one site to
                        another are called:
                                a. insertion sequences
                                b. palindromic sequences
                                c. transposons
                                d. muons

                    A term coined to describe a bacterium which requires a growth factor in order to grow is:
                                a. auxotroph
                                b. palindrome
                                c. heterotroph
                                d. apoptosis

                    When the F plasmid replicates as part of the chromosome, the progeny cells are:
                                a. Hfr cells
                                b. F-cells
                                c. F plasmid cells
                                d. heterogeneous nuclear RNA cells

                    The DNA enzyme which reacts with specific short sequences of nucleotides in foreign
                        DNA and breaks the phosphate backbone at these sites is called:
                                a. ribonuclease
                                b. transferase
                                c. restriction endonuclease
                                d. DNase

                    The process of removing or killing all microorganisms in or on a material is termed:
                                a. sterilization
                                b. disinfection
                                c. sanitation
                                d. obfuscation

                    The most resistant of the vegetative organisms to the action of germicides is:
                                a. Streptococcus pneumonia
                                b. Salmonella typhi
                                c. Bacillus cerus
                                d. Mycobacterium tuberculosis

                    An important component of the endospore which aids in its resistance to destruction is:
                                a. dipicolinic acid
                                b. chromatin
                                c. microtubules
                                d. histone

 
 

SCI 252 - Medical Microbiology
Examination #2

 
Pts.    Question

5          1. Define each of the following terms:
                    a. tropism
                    b. viroid
                    c. secondary response
                    d. attenuation
                    e. iatrogenic disease

5          2. Provide the names of three bacteria that exemplify the diversity of the
                    microbial world.

10        3. Choose and answer ONE of the following:
                    a. Describe the three phases of the common viral reproductive
                                cycle,         OR
                    b. Describe the initiation of viral infection,    OR
                    c. Differentiate the five types of viral infection.

10        4. Compare and contrast the viral multiplication schemes of RNA and
                    DNA viruses.

10        5. Choose and answer ONE of the following:
                    a. Describe antigen processing,    OR
                    b. Describe the cellular process of inflammation.

10        6. Choose and answer ONE of the following:
                    a. Describe the five classes of immunoglobulin,    OR
                    b. Diagram an IgG antibody in detail.

10        7. Choose and answer ONE of the following:
                    a. Explain how clonal expansion affects memory cell development,
                                OR
                    b. Design an epidemiologic study to demonstrate herd immunity.
                                
10        8. Differentiate therapeutic dose and therapeutic index.

10        9. Choose and answer ONE of the following:
                    a. What are the steps that can be taken to limit the development of
                            resistance? OR
                    b. Why are narrow spectrum antibiotics chosen before broad
                            spectrum antibiotics? Give an example of each and its
                            range.

20        10. Answer each of the following one point questions:
                    Which of the following lists the essential viral components?
                        a. genome, capsid
                        b. nucleic acid, capsid, envelope
                        c. genome, virion, envelope
                        d. capsid, nucleic acid, capsule

                    The protein projections on the surface of viruses which function in attachment to the host
                        cell are called-
                        a. suckers
                        b. pili
                        c. hooks
                        d. spikes

                    The infectious agent that is devoid of nucleic acid but not protein is
                        a. prion
                        b. viroid
                        c. virus
                        d. capsid

                    A key feature of all viral infections is the
                        a. union of the nucleic acid with the host cell membrane
                        b. separation of the host nucleic acid from its cytoplasm
                        c. removal of the host receptor site from the viral program
                        d. separation of nucleic acid from protein coat

                    An exit method used by viruses which does not destroy tyhe host cell is
                        a. lysis
                        b. inversion
                        c. budding
                        d. excising

                    Proteins produced by the body in response to invasion by a foreign substance which
                        interacts specifically with that substance-
                        a. antibodies
                        b. antibiotics
                        c. peroxides
                        d. toxins

                    Which of the following is not involved in the immune response?
                        a. neutrophils
                        b. macrophages
                        c. erythrocytes
                        d. lymphocytes
 
                    The cells primarily responsible for defense of the body against microorganisms are the
                        a. leukocytes
                        b. erythrocytes
                        c. platelets
                        d. osteocytes

                    A group of interacting serum proteins which provide a non-specific defense mecahnism is
                        a. complement
                        b. interferon
                        c. glycoproteins
                        d. chemotoxic agents

                    The attraction of leukocytes to the area of inflammation is referred to as
                        a. parasitism
                        b. infection
                        c. chemotaxis
                        d. premeditation

                    A term synonymous with antibody is
                        a. antigen
                        b. immunoglobulin
                        c. protein
                        d. enzyme

Which of the following immunoglobulins is a pentamer?
    a. IgA
    b. IgD
    c. IgE
    d. IgM
    e. IgE

The Y-shape of the IgG molecule is held together by
    a. disulfide bonds
    b. hydrogen bonds
    c. nitrogen bonds
    d. complement

Which of the following antibodies is found primarily in external secretions like milk and
    saliva?
    a. IgD
    b. IgG
    c. IgA
    d. IgM
    e. IgE

Another term by which helper T cells are known is
    a. CD1+
    b. CD2+
    c. CD3+
    d. CD4+
 
During clonal expansion, memory cells are produced by
    a. both T and B cells
    b. neither T nor B cells
    c. B cells only
    d. T cells only

The microorganisms that are found on the body but do not produce obvious harmful effects
    are known as
    a. normal fauna
    b. normal flora
    c. transient flora
    d. feral flora

Which of the following can suppress the growth of normal flora which then allows
    pathogens to colonize the host?
    a. peroxides
    b. antibiotics
    c. toxins
    d. fatty acids

Establishment of microbial growth on or in the host is referred to as
    a. colonization
    b. commensalism
    c. pathogenic
    d. parasitism

A disease which rapidly spreads to include most of the world population is called a(n)
    a. epidemic
    b. pandemic
    c. endemic
    d. zoonosis

The possible sources of an infectious disease agent is referred to as its
    a. vector
    b. transmission pool
    c. fomite
    d. reservoir

 

SCI 252 – MICROBIOLOGY
EXAMINATION #3
 

Pts.    Question

5        1.  Choose and answer ONE of the following:
                a. What causes folliculitis, furuncles, and carbuncles, and how are they treated?
                            OR
                b. How are pyoderma and impetigo spread?
                            OR
                c. How are microorganisms involved in acne?
                            OR
                d. What organisms are frequently found in burn infections?
 

10        2. Match the following:
                German measles                                                                     a. varicella
                measles                                                                                  b. vaccinia virus
                chickenpox                                                                              c. rubeola
                Koplik’s spots                                                                          d. papillomavirus
                warts                                                                                       e. zoster
                shingles                                                                                   f. rubella
                cowpox
                SSPE

10        3. Choose and answer ONE of the following:
                a. How is strep throat diagnosed and treated?    OR
                b. How is diptheria diagnosed, treated, and prevented?    OR
                c. What agents cause colds and how are they transmitted?

10        4. Choose and answer ONE of the following:
                a. What causes tuberculosis and why is it a global problem?    OR
                b. How is tuberculosis transmitted and what populations are most susceptible?

10        5. List the special characteristics of the Epstein-Barr virus. What diseases does it cause and
                how are they diagnosed and treated?

10        6. Choose and answer ONE of the following:
                a. List the characteristics of rabies. What treatment is available?    OR
                b. Why is rabies difficult to control and what measures are available?

10        7. Choose and answer ONE of the following:
                a. Contrast the tuberculoid and lepromatous forms of Hansen’s disease,     OR
                b. What is the cause of tetanus and how do humans acquire it?

10        8. Design and justify a sexually transmitted diseases control program for Lewiston, ME.
 
10        9. Match the following:
                ___ chancroid                     a. herpes
                ___ gonorrhea                     b. papilloma virus
                ___ syphilis                         c. Esherichia coli
                ___ Donovan bodies             d. Haemophilus
                ___ yaws                             e. Neisseria
                ___ toxic shock syndrome     f. Gardnerella
                ___ buboes                          g. Treponema
                ___ chancre                         h. Staphylococcus
                ___ clue cells                        i. Chlamydia
                ___ bejel                               j. Calymmatobacterium
                ___ genital warts
                ___ whitlow
                ___ cause of most UTIs